pSAH v2 vector map

pSAH v2

Protein Expression
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Description

Expression vector designed for Golden Gate cloning of an optional secretion signal and ORF. The BsaI stuffer fragment contains a Pichia pastoris ARS, allowing the unmodified plasmid to be used directly as a transformation control. Linearized with PmeI for integration at the AOX1 promoter for methanol-inducible expression. Lox sites enable the removal of vector backbone and antibiotic resistance marker with pArsTG-Cre.

Specifications

Promoter
AOX1Inducible
Selection Marker
Hygromycin(300 μg/mL)
Vector Size
5,127 bp
LoxP Sites
Yes

About AOX1 Promoter

Full name: Alcohol oxidase 1

Strength: STRONG

Type: Inducible

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