Expression Vectors
High-quality vectors optimized for protein expression in Pichia pastoris
Heterologous Protein Expression
pJAG v2
Expression vector designed for Golden Gate cloning of an optional secretion signal and ORF. The BsaI stuffer fragment contains a Pichia pastoris ARS, allowing the unmodified plasmid to be used directly as a transformation control. Linearized with PmeI for integration at the AOX1 promoter for methanol-inducible expression.
pJAH v2
Expression vector designed for Golden Gate cloning of an optional secretion signal and ORF. The BsaI stuffer fragment contains a Pichia pastoris ARS, allowing the unmodified plasmid to be used directly as a transformation control. Linearized with PmeI for integration at the AOX1 promoter for methanol-inducible expression.
pJAN v2
Expression vector designed for Golden Gate cloning of an optional secretion signal and ORF. The BsaI stuffer fragment contains a Pichia pastoris ARS, allowing the unmodified plasmid to be used directly as a transformation control. Linearized with PmeI for integration at the AOX1 promoter for methanol-inducible expression.
pJAZ v2
Expression vector designed for Golden Gate cloning of an optional secretion signal and ORF. The BsaI stuffer fragment contains a Pichia pastoris ARS, allowing the unmodified plasmid to be used directly as a transformation control. Linearized with PmeI for integration at the AOX1 promoter for methanol-inducible expression.
pJ_G v2
Expression vector designed for Golden Gate cloning of a promoter, optional secretion signal, and ORF. The BsaI stuffer fragment contains a Pichia pastoris ARS, allowing the unmodified plasmid to be used directly as a transformation control.
pJ_H v2
Expression vector designed for Golden Gate cloning of a promoter, optional secretion signal, and ORF. The BsaI stuffer fragment contains a Pichia pastoris ARS, allowing the unmodified plasmid to be used directly as a transformation control.
pJ_N v2
Expression vector designed for Golden Gate cloning of a promoter, optional secretion signal, and ORF. The BsaI stuffer fragment contains a Pichia pastoris ARS, allowing the unmodified plasmid to be used directly as a transformation control.
pJUsG v2
Expression vector designed for Golden Gate cloning of an optional secretion signal and ORF. The BsaI stuffer fragment contains a Pichia pastoris ARS, allowing the unmodified plasmid to be used directly as a transformation control. Linearized with AleI/EcoRV for integration at the UPP promoter for constitutive expression.
pJUsH v2
Expression vector designed for Golden Gate cloning of an optional secretion signal and ORF. The BsaI stuffer fragment contains a Pichia pastoris ARS, allowing the unmodified plasmid to be used directly as a transformation control. Linearized with AleI/EcoRV for integration at the UPP promoter for constitutive expression.
pJUsN v2
Expression vector designed for Golden Gate cloning of an optional secretion signal and ORF. The BsaI stuffer fragment contains a Pichia pastoris ARS, allowing the unmodified plasmid to be used directly as a transformation control. Linearized with AleI/EcoRV for integration at the UPP promoter for constitutive expression.
pJUsZ v2
Expression vector designed for Golden Gate cloning of an optional secretion signal and ORF. The BsaI stuffer fragment contains a Pichia pastoris ARS, allowing the unmodified plasmid to be used directly as a transformation control. Linearized with AleI/EcoRV for integration at the UPP promoter for constitutive expression.
pJ_Z v2
Expression vector designed for Golden Gate cloning of a promoter, optional secretion signal, and ORF. The BsaI stuffer fragment contains a Pichia pastoris ARS, allowing the unmodified plasmid to be used directly as a transformation control.
pSAH v2
Expression vector designed for Golden Gate cloning of an optional secretion signal and ORF. The BsaI stuffer fragment contains a Pichia pastoris ARS, allowing the unmodified plasmid to be used directly as a transformation control. Linearized with PmeI for integration at the AOX1 promoter for methanol-inducible expression. Lox sites enable the removal of vector backbone and antibiotic resistance marker with pArsTG-Cre.
pSAN v2
Expression vector designed for Golden Gate cloning of an optional secretion signal and ORF. The BsaI stuffer fragment contains a Pichia pastoris ARS, allowing the unmodified plasmid to be used directly as a transformation control. Linearized with PmeI for integration at the AOX1 promoter for methanol-inducible expression. Lox sites enable the removal of vector backbone and antibiotic resistance marker with pArsTG-Cre.
pSAZ v2
Expression vector designed for Golden Gate cloning of an optional secretion signal and ORF. The BsaI stuffer fragment contains a Pichia pastoris ARS, allowing the unmodified plasmid to be used directly as a transformation control. Linearized with PmeI for integration at the AOX1 promoter for methanol-inducible expression. Lox sites enable the removal of vector backbone and antibiotic resistance marker with pArsTG-Cre.
pS_H v2
Expression vector designed for Golden Gate cloning of a promoter, optional secretion signal, and ORF. The BsaI stuffer fragment contains a Pichia pastoris ARS, allowing the unmodified plasmid to be used directly as a transformation control. Lox sites enable the removal of vector backbone and antibiotic resistance marker with pArsTG-Cre.
pS_N v2
Expression vector designed for Golden Gate cloning of a promoter, optional secretion signal, and ORF. The BsaI stuffer fragment contains a Pichia pastoris ARS, allowing the unmodified plasmid to be used directly as a transformation control. Lox sites enable the removal of vector backbone and antibiotic resistance marker with pArsTG-Cre.
pSUsH v2
Expression vector designed for Golden Gate cloning of an optional secretion signal and ORF. The BsaI stuffer fragment contains a Pichia pastoris ARS, allowing the unmodified plasmid to be used directly as a transformation control. Linearized with AleI/EcoRV for integration at the UPP promoter for constitutive expression. Lox sites enable removal of vector backbone and antibiotic resistance marker with pArsTG-Cre.
pSUsN v2
Expression vector designed for Golden Gate cloning of an optional secretion signal and ORF. The BsaI stuffer fragment contains a Pichia pastoris ARS, allowing the unmodified plasmid to be used directly as a transformation control. Linearized with AleI/EcoRV for integration at the UPP promoter for constitutive expression. Lox sites enable removal of vector backbone and antibiotic resistance marker with pArsTG-Cre.
pSUsZ v2
Expression vector designed for Golden Gate cloning of an optional secretion signal and ORF. The BsaI stuffer fragment contains a Pichia pastoris ARS, allowing the unmodified plasmid to be used directly as a transformation control. Linearized with AleI/EcoRV for integration at the UPP promoter for constitutive expression. Lox sites enable removal of vector backbone and antibiotic resistance marker with pArsTG-Cre.
pS_Z v2
Expression vector designed for Golden Gate cloning of a promoter, optional secretion signal, and ORF. The BsaI stuffer fragment contains a Pichia pastoris ARS, allowing the unmodified plasmid to be used directly as a transformation control. Lox sites enable the removal of vector backbone and antibiotic resistance marker with pArsTG-Cre.