pSUsH v2 vector map

pSUsH v2

Protein Expression
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Description

Expression vector designed for Golden Gate cloning of an optional secretion signal and ORF. The BsaI stuffer fragment contains a Pichia pastoris ARS, allowing the unmodified plasmid to be used directly as a transformation control. Linearized with AleI/EcoRV for integration at the UPP promoter for constitutive expression. Lox sites enable removal of vector backbone and antibiotic resistance marker with pArsTG-Cre.

Specifications

Selection Marker
Hygromycin(300 μg/mL)
Vector Size
5,273 bp
LoxP Sites
Yes
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